Praktikum Enzym

 

ENZYME II

 

1.1.            Goal

f  To know the effect of substrate concentration on the activity of catalase enzyme.

 

f   

 

 

1.2.            Basic Theories.

 

Because enzymes are specific in action, certain classes of enzymes will act on certain substrates only. For example, carbohydrases digest sugars and starches; lipases break down lipids; and proteses split up proteins.

 

 

1.3.            Tools and Materials

a.        Tools

j  Dropper pipette

j  Ruler

j  Reaction tubes and rack/holder

j  Measuring pipette 10 ml with sucker bulb

j  Scalpel or cutter

j  Marker or labels

b.        Materials

b  Chicken liver

b  Distilled water

b  Hydrogen peroxide (H₂ O₂)

1.4.            Working Procedure

Write down the working procedure explained by your teacher in the column below !

 

Prepare 5 reaction tube and place them on the rack, and give labels of numbers 1 up to 5 Put H2O2 and aquades in tube, according the table below !

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

1.5.            Observation Result

 

 

 

 

 

 

 

 

 

 

 

 

 

1.6.            Questions

1.6.1.1.     What kind of enzyme does chiken liver have ?

 

 

1.6.1.2.    What is the function of  the enzyme?

 

 

1.6.1.3.    What is the functions of hydrogen peroxside